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Tobacco smoke is a mixture of over 4700 chemical compounds including oxidants, which are present at high concentration [20]

Unlike the self-polymerization approach, requiring high alkalinity and long reaction times, the enzymatic method achieves fast polymerization under mild conditions, which broadens its range of substrates and applications (Magalhes et al., 2022)

TKL2, transketolase 2

The treatment with Ru(II)-OH or NP2 showed a strong green fluorescence signal throughout the cell, indicative of the depletion of O 2 and therefore the O 2 consumption through the catalyst

Patients typically receive injections once or twice weekly for the first two weeks, followed by monthly maintenance doses, making the treatment cost-effective for those requiring consistent B12 supplementation