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(Diagram illustrating the mitochondrial genome, the production of the MOTS-c peptide within the mitochondria, and its subsequent translocation to the cytoplasm and nucleus to influence cellular processes.) The Key Pathway: How MOTS-c Activates AMPK The primary MOTS-c mechanism of action involves the robust activation of a critical enzyme: 5 AMP-activated protein kinase, or AMPK

AOD9604 did not stimulate the production of IGF-1 in any of the systems studied, and elicits its fat-reducing effects without inducing insulin resistance a key pharmacological distinction from intact hGH that makes it a uniquely clean research tool for studying lipolytic mechanisms in isolation

Figure S4: Respiratory burst measured by lucigenin dependent chemiluminescence is not quenched by addition of 5mM N-acetyl cysteine (NAC) or 5M sulphoraphane (SFN) when added at the peak of neutrophil respiratory burst