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Follow-ups were conducted at 1, 3, 6, and 12 months post-treatment

4) Kinetic studies of the partially purified GGT isoenzyme-I: Optimum substrate concentration and Km: The concentration of the basic materials was studied against the reaction speed of GGT iso-enzyme-I partially separated from the ionic exchange column until the ideal concentration [2 mM] (Figure 3(a) & Figure 3(b)), respectively

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