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Analytical SEC Analytical SEC was performed by applying 20 g of each anti-NIP IgG variant to a Superdex 200 Increase 3.2/300 analytical SEC column (Cytiva Lifesciences) at a flow rate of 0.05 mL/min using an AKTA FPLC instrument (Cytiva Life sciences)

The statistical identification of glucose-responsive genes was conducted using R (v1.1.456) and EdgR (3.30.3) package 90

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Basic conditions (NaOH) are also required for the reticulation of CD with EPI (Olteanu et al., 2014

Intensive Protocol In more intense anti-aging regimes, some administer 20 mg daily (in two 10 mg injections) for 10 days, typically once yearly