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By inhibiting lipogenesis, enhancing fatty acid oxidation, improving glucose utilization, and supporting vascular, neural, cardiac, and cellular metabolic stability, capsaicin-activated AMPK represents a key mechanism by which capsaicin promotes metabolic homeostasis under conditions of nutrient excess and cardiometabolic stress

To improve neuronal survival and promote spine maturation, neuron-astrocyte co-culture systems were developed and refined where astrocytes provide tropic support ( Several groups have used modifications of this model to study the impact of AD stimulators, and prion disease-related neurodegeneration on the neuronal dendritic spine number and density ( in vitro platform of primary mouse neurons, following a modified bottom-up approach, to model the process of neuronal synaptic degeneration in the context of AD

This dual-compound approach facilitates examination of synergistic pathway interactions while maintaining precise experimental control over individual molecular mechanisms through separate component analysis