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doi:10.1016/j.chom.2020.09.016 14

Thromb J 18:19 Ben Ismail TH, Ali BH, Bashir AA (1994) Influence of iron, deferoxamine and ascorbic acid on gentamicin-induced nephrotoxicity in rats

Acid-stressed mAb_A showed a more than 1.5 times stronger signal than other types (mAb_B, mAb_C, and pAb)

So thats something to consider

The hemolysate is losing the normal red pigmentation of the hemoglobin into brown color due to iron effect with different concentrations concerning time (5 minutes)
