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Check the date COAs should be recent (within 6 months)
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During the expression of uncultured microbes, protein expression faced difficulties
Cells were manually scraped, spun at a 100 g for 5 min, the pellets washed with PBS and then homogenized in a Tris salt buffer, transferred to ultracentrifuge tubes and spun at 163, 200 g and 4 C for 30 min to extract the soluble fraction
Hydrolysis still occurs, but so slowly that the peptide maintains therapeutic potency for months
