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The worms were permeabilized and fixed after being resuspended in 400 l of PBS to which an equal volume of 2 MRWB (160 mM KCl, 40 mM NaCl, 14 mM Na 2 EGTA, 1 mM spermidine-HCl, 0.4 mM spermine, 30 mM Na-PIPES pH 7.4, 0.2% -mercaptoethanol) buffer containing 4% paraformaldehyde (PFA) was added

B12 supports methylation reactions that convert homocysteine back to methionine

This is why dauer larvae die from the rapid and premature consumption of energy stores after the loss of the aak-2 function [122]

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USA 112 , E6562E6570 (2015)