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TB-500 also appears to be generally well-tolerated, but again, safety data derived from adequately powered clinical trials is lacking

After blocking in 5% skim milk/phosphate-buffered saline supplemented with .1% Tween 20 (PBS-T), membranes were incubated with the primary antibody overnight at 4C and then incubated with the secondary antibody for 1 h at room temperature

Peak concentration for brimonidine is 1 to 4 hours after instillation and 1 to 3 hours for timolol after instillation

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