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The LC treated monolayers of VSMCs in 25-cm 2 culture flasks were rinsed twice with 1 mmol/L EDTA in PBS, lysed with Triton lysis buffer (50 mmol/L TrisHCl, pH 8.0, containing 150 mmol/L NaCl, 1% Triton X-100, 0.02% sodium azide, 10 mmol/L EDTA, 10 mg/mL aprotinin and 1 mg/mL aminoethylbenzenesulfonyl fluoride) and then saved after centrifugation