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endpoints A and Z) added with 498 l of RPMI 1640 culture medium (Sigma-Aldrich, Saint Louis, Missouri) and 2 l of pH 5 citrate-phosphate buffer excipient (Merck, Darmstadt, Germany)

In practice for clinicians, it is important to note that certain complications of vitamin B12 deficiency, in particular psycho-neurological and hematological complications are potentially serious, as seen in Table 1, and therefore require investigation in all patients who present with vitamin or nutritional deficiency