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Many of the alterations in enhancer methylation lies in the genes regulating the expression of cell cycle regulatory proteins like cyclin-dependent kinases (CDKs)

Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)

Variation in colonization, ADP-ribosylating and vacuolating cytotoxin, and pulmonary disease severity among mycoplasma pneumoniae strains

Synthetic antioxidants Synthetic antioxidants have been developed to have a standard antioxidant activity measurement system and to compare with natural antioxidants that are incorporated into food