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Supplementary information The online version contains supplementary material available at 10.1038/s41467-024-45808-9

The eCB name is obviously derived from the term cannabis, and this is due to the fact that CB1 is also the primary CNS molecular target for 9 -tetrahydrocannabinol ( 9 THC), the major psychoactive ingredient in preparations of Cannabis sativa , which acts as a partial agonist at these receptors

Research Protocol Development Study Design Considerations : When planning epithalon research, consider these essential elements: Primary endpoints : Clearly define measurable outcomes Control groups : Include appropriate placebo or vehicle controls Dosing schedules : Plan administration timing and frequency Sample collection : Establish biospecimen collection protocols Duration planning : Account for both acute and chronic effects Documentation Requirements : Detailed protocol documentation Informed consent procedures (where applicable) Data collection standardization Quality control checkpoints Adverse event reporting systems Laboratory Setup and Equipment Essential Equipment : Analytical balance for precise measurements Refrigerated centrifuge for sample processing -80C freezer for long-term storage Sterile laminar flow hood for preparation Appropriate syringes and filters for reconstitution Safety Considerations : Personal protective equipment protocols Waste disposal procedures for peptide materials Emergency response plans Training requirements for research staff Documentation of safety protocols Data Collection and Analysis Biomarker Selection : Choose appropriate markers based on research objectives: Aging-related : Telomere length, oxidative stress markers Cellular function : Mitochondrial activity, protein synthesis Immune function : Cytokine profiles, immune cell counts Metabolic markers : Glucose tolerance, lipid profiles Statistical Considerations : Power analysis for sample size determination Appropriate statistical tests for data types Multiple comparison corrections Time-series analysis for longitudinal studies Effect size calculations for clinical significance Researchers can benefit from exploring comprehensive research approaches that integrate multiple methodologies for robust study design

Depending on the location where the temperature is measured, different values are obtained

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