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For rescue experiments, 50100 ng/l of acs-2::gfp was injected into nhr-49(nr2041) and WT animals along with 50 ng/l of pRF4 rol-6(su1006)
This can lead to smoother results and less damage to the surrounding tissues
Air means oxygen
Stock solutions were diluted into 40 L of a 50:50 mixture of solvent: water or 40 L of solvent only, as indicated, then applied topically to NGM 3.5 cm diameter petri dish plates (4 mL NGM agar volume) seeded with E
