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While freezing did not impact the concentrations of NAD + , NADH, GSH, or GSSG, it triggered the conversion of NADPH to NADP + , with about 508% of NADP + originating from NADPH oxidation (Supplementary Figure 1m-r)

L-RZ, S-SL and W-QZ contributed to the acquisition, analysis, and interpretation of literature for the work

The results showed that Tanzeum bound with slightly faster association and dissociation rates than GLP-1 WT, resulting in a 1.5-fold difference in binding affinity to the receptor (Tanzeum: 76.1 nM

The mouse has a 5-minute intertrial interval in its home cage

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