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& Jewess, P

To assess the efficiency of different elution strategies, the BLI method was used in which APC was captured by HS02-52G aptamer immobilized on the SAX-biosensors and the dissociation step was performed in the binding buffer containing either 1 M NaCl or 5 mM EDTA

Cells were cultured in a 5% CO 2 incubator at 37C, and the medium was changed every day

It also performs Thiol Buffering, which maintains the reduced state of sulfhydryl groups on essential proteins, preventing oxidative damage and preserving protein function

Nevertheless, proteins are likely to be the primary target for these oxidants, given their abundance in the cell and their high reactivity