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PMID: 32298162

20% of the over 60s are deficient in B12, and 11% of those who follow a vegan diet are also deficient The most common cause of Vitamin B12 deficiency is pernicious anaemia, however, it is estimated that 20% of the over 60s are deficient in B12, and 11% of those who follow a vegan diet are also deficient

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Western blotting Cells were harvested on DIV6 using M-PER lysis buffer (Thermo Fisher Scientific) supplemented with protease and phosphatase inhibitors (Roche, Basel, Switzerland)