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The critical points were checked to have near-zero gradients, and zero (for the minima) or one (for the transition states) imaginary frequencies evaluated at the level of optimization

Delafontaine P, Song YH, Li Y: Expression, regulation, and function of IGF-1, IGF-1R, and IGF-1 binding proteins in blood vessels

This suggests that the extra carbohydrate intake in multiple times a day particularly damages -cells

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