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For intracellular cytokine, staining cells were fixed with BD Cytofix Cytoperm (BD Biosciences) for 20 min at 4 C, washed twice with 1x saponin buffer (0.5% (w/v) saponin [Carl Roth], 2 mM EDTA, 2% FCS, in PBS) and stained for IFN (see Table 1) overnight at 4 C in 1 saponin buffer

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Thus: The percentage inhibition (P.I.) of a given drug was calculated by the equation of Hano et al

Alterations in the expressions were determined by 2 -CT , taking -actin as inner regulator

However, it characterized the paradox of timolol concomitant to epinephrine to enhance the ocular hypotensive effects in many patients [66]