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It is an essential nutrient which is water soluble

It directly stimulates pro-opiomelanocortin/cocaine-and-amphetamine-regulated transcript (POMC/CART) neurons and indirectly inhibits neuropeptide-Y/agouti-related protein (NPY/AgRP) neurons through GABA neurotransmission, reducing appetite and promoting weight loss [40]

To test if the lowered circulating insulin levels in HF-SAAR mice was contributing to the metabolic adaptations to SAAR, a subcutaneous, sustained-release insulin pellet was implanted in HF-SAAR mice to raise insulin

PMID: 25380454

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