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Cells were lysed in PBS/Triton X-100 (0.5%) for 60 min

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Screening techniques for DPN, such as the 10-g monofilament and assessment of touch, vibration sense, and ankle reflexes, have limitations, primarily detecting advanced neuropathy and missing early stages, especially those associated with small fiber abnormalities and neuropathic pain [14, 17,18,19,20,21,22]

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Front Pharmacol (2022) 13:806470

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