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Chemicals Metformin and phenformin were dissolved in water with the stock solutions as 1 M and 0.1 M respectively and were added to OP50 or HT115 bacteria seeded plates 24 hours before adding worms

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The phase II and III European studies involved 16mg melanotan I implants for a treatment period of up to four months [8, 9, 10, 11, 12, 13, 14, 15]

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