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The most apical structures, the TJs, are the rate-limiting factor for the epithelial paracellular permeation of molecules

Side effects are rare and usually mild, such as slight redness or swelling at the injection site

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In order to accomplish this, water must flow upward at a rate high enough to fluidize the media bed, which widens the gap between the media grains and causes the media to "expand," or take up more space