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FAM-Peptide and protein stock solutions were diluted to the desired concentration with assay buffer (10 mM HEPES, pH 7.4, 1 mM CaCl 2 , 150 mM NaCl, 0.1% Pluronic)
Am J Psychiatry 162(2):214227
Although, more recently, the silicon model study of the in vivo nutrient microenvironment of degenerative intervertebral disc has been found nutrient concentrations may increase by a reduction in diffusional distance due to reduced disc height and vasculature ingrowth 57
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