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Effects of green tea polyphenol ()-epigallocatechin-3-gallate on newly developed high-fat/Western-style diet-induced obesity and metabolic syndrome in mice

Mice and diets All animal care and use procedures were approved by the Institutional Animal Care and Use Committee of Capital Medical University and were conducted in a specific-pathogen-free facility at Anzhen Hospital, Capital Medical University, Beijing, China

For intracellular staining, cells were fixed with IC fixation buffer (Fisher Scientific), permeabilized and incubated in the dark with a fluorochrome-labelled antibody cocktail in 1 permeabilization buffer (Fisher Scientific) for 30 min at room temperature

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