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Cells were soaked in the PBS supplemented with 10 mM Tris HCl (pH 7.5), 10% glucose, 10 mM cysteamine, 50 mM 2-mercaptoethanol, 2.5 mM protocatechuic acid, 2 mM cyclooctatetraene, and 50 mM protocatechuic dioxygenase just before the observation
Biederman J

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