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To date, the roles of ZO proteins in TJs are not fully understood

Bacterial-derived components have also been reported to directly affect tight junction assembly

But the authors themselves are explicit that this was an in vitro study using human cell lines in 2D cell cultures, that qPCR measures only average telomere length across chromosomes, that only a handful of cell lines were tested, and that human trials remain necessary before clinical application. [2] A single 2025 replication strengthens the cell-biology claim

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"Humans from all eras have been relying on this design," says Adrian Bejan, a mechanical engineer at Duke University