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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich

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The samples were fixed in 10% formalin for 48 h, followed by decalcification in 10% ethylenediaminetetraacetic acid (EDTA, 25102-12-9, Sigma-Aldrich) for 4 weeks

However, pregnant women using high doses of Vitamin B6 over an extended period may risk causing drug dependence syndrome in newborns

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