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Cutting edge: VacA, a vacuolating cytotoxin of Helicobacter pylori , directly activates mast cells for migration and production of proinflammatory cytokines

Finally, the filtered cell lysate was centrifuged at 4 C for 10 min at 10,000 g

(2005) 67:S83S91

doi: 10.1093/ijnp/pyz052 [DOI] [PMC free article] [PubMed] [Google Scholar] 169

The 3D cell culture Matrigel (Corning, #354234) was thawed slowly on ice and mixed with suspended cells (1 10 3 cells) at different concentrations