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JiangJXiaoHWuKYuZRenYZhaoYet al

Methodological aspects, such as differences in sleep deprivation protocols, brain regions analyzed, and experimental models, along with the observation that the brain does not appear to be significantly damaged by oxidative stress after sleep deprivation, have led to search for signs of oxidative stress in other organs
This green synthesis pathway reduces production energy requirements and toxic byproducts

Song et al., 2013

The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system
