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Description
It is when the mutation load exceeds this threshold, we see metabolic dysfunction and clinical symptoms associated with a disease state (Niyazov et al., 2016)
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In this shuttle, NADPH is formed at the expense of one ATP used by pyruvate carboxylase and one NADH utilized by malate dehydrogenase

GPX4 reverse, 5- CCGAACTGGTTACACGGGAA-3

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