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Briefly, fecal samples were suspended in sterile distilled water by vigorous shaking, and the supernatant was transferred to a 0.5 ml tube containing glass beads (0.1 mm diameter), before being spun at 5,000 rpm for 3 min in a mini-bead beater (BioSpec Products, Bartlesville, OK, USA)

Role of acetyl-L-carnitine in the treatment of cognitive deficit in chronic alcoholism 29

However, the role of miR-335 in triggering lipid metabolism still remains unknown