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However, it is not known whether vitamin B 12 supplementation, in addition to folic acid supplementation, may be beneficial in the prevention of NTD (62) randomized controlled trials are needed to address this question

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12, 16, 18 A high degree of synergistic enhancement of L-PAM cytotoxicity in the presence of BSO was observed in multidrug-resistant neuroblastoma cell lines, including those that were established at relapse after myeloablative therapy with L-PAM and lines highly resistant to L-PAM due to loss of p53 function, especially at concentrations of L-PAM that were myeloablative

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A second round of PCR was performed on 1 l of step 1 PCR product using Nextera i5 and i7 dual-index library primers (Illumina)