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Several considerations should be accounted for, besides accurate mass (AM) and retention time (RT), which includes the elimination of background signals, deisotoping, recognition of adduct peaks in spectra, and MS/MS fragmentation pattern compared to databases or internal standards and the matching degree estimated (Figure 2)
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In both melanoma (253, 254) and breast cancer (255, 256), CLND did not show an overall survival benefit compared to SLNB alone in early-stage cancer patients
