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Many studies have been conducted to determine whether there is an association between the C677T polymorphism and an increased risk of cardiovascular disease, hypertension, diabetes, and overweight/obesity

The first-line test is serum vitamin B12 measurement , with levels below 148 pmol/L generally considered deficient, though the exact threshold varies between laboratories

B12 absorption is a complex process B12 found in food follows a complex, two-step pathway in order to be absorbed into the bloodstream

After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days

Cambridge: Chapman Hall