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In this situation, the O 2 -O 3 AHT may have a role in immune system rebalance and prevention of endothelial damage and vascular thrombosis by the HO-1 production, via Nrf2 activation

Reaction mix with cell lysate and 1 mM ATP, but no F6P, was monitored as blank control

RNA interference We transfected shRNA into extracted epithethial cells using Lipofectamine 3000 (#L3000008, Thermo Fisher, USA) according to the manufacturers instruction

In Figure 3, as an additional analysis by Bonferroni, we verified the differences in glutathione levels according to the degree of suicide risk, observing a significant association between the differences in glutathione means in the group of women with moderate to high risk compared to the reference group (without risk of suicide)