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Besides being a potential source of more reactive free radicals via Fenton reaction, physiological generation of H 2 O 2 fulfills a second messenger role and can be transported across membranes by aquaporins, a family of proteins that act as peroxiporins (Sies, 2014)
J Exp Med 180:19851988
doi: 10.1046/j.1471-4159.2001.00374.x
PMID: 9862719
GST pulldowns or immunoprecipitation are the most common version of this type of experiment, but you can perform analogous isolation experiments with other protein tags including his-tags and strep-tags (Figure 7)
