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Strains for LpoB complementation titers and growth curve assays were prepared as above, through CRIM-integration of LpoB variants under the control of the IPTG-inducible lac promoter (Plac) into the background of a lpoB deletion and ponA depletion strain (CB4) 30 and selection with 10 g/mL tetracycline (tet 10 ) on LB-agar supplemented with 0.2% arabinose

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It is worth noting that intervention in the metabolic pathway of TMAO may be an underlying therapeutic target of AF

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