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J Pharm Pharmaceut Sci, 6: 327-333, 2003

GST-Pull Down Technology Introduction to protein interactions The functioning of biological systems is inextricably linked to protein-protein interactions, and important biological processes such as DNA synthesis, gene transcriptional activation, protein translation, modification and localization, and information transfer all involve the role of protein complexes

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For enzymatic reduction, either purified enzymes or CLs were added and the mixtures were incubated supplement with 500 M DTT at 37 C

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