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At higher doses, it is also absorbed by passive diffusion across the gut wall, independently of intrinsic factor

The resulting supernatant was then measured for its absorbance at 532 nm, and standard curves were created using Tetramethoxypropane (obtained from Sigma, USA)

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However, because complexes that contain inhibitor (ESI) are incapable of progressing to reaction products, the effect of a noncompetitive inhibitor is to reduce the concentration of ES complexes that can advance to product