l-glutathione gsh reduced Simplify your Glutathione Measurements – Arbor Assays Explore its role in cellular health GSH- Reduced L-Glutathion, 60 capsules

We therefore omitted these two cysteine mutants from these panels, as no functional readout was available to assess mutational effects

Talanta 253 , 123944 (2023)

The assay is based on a bioluminescence reaction between an enzyme, luciferase, and a substrate, luciferin

PCa cell lines LNCaP, 22RV1, DU145, PC3, and 293T were purchased from the Chinese Academy of Sciences, Shanghai Academy of Sciences, and C4-2 was purchased from the American Type Culture Collection (ATCC)

Endogenous co-immunoprecipitation LnCaP cells were lysed and scraped in cold lysis buffer (20 mM Tris-HCl, Ph 8, 130 mM NaCl, 1% Triton X-100, 1 mM DTT, 10 mM NaF, 1 complete EDTA-free protease cocktail tablet (Roche), Phosphatase cocktail inhibitors II and IV (Sigma))