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Preparation of mouse anti-GST sera To prepare mouse anti-GST sera, 6 mice for each GST were used and each mouse was injected intraperitoneally with 0.5 ml of 200 g/ml of recombinant GST completely mixed with an equal volume of Freunds Complete Adjuvant (Sigma-Aldrich) to give each mouse 100 g recombinant protein

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In cancer, ROS act as a double-edged sword: while some cancer therapies are based on triggering intracellular ROS to the point of cellular destruction 5 , ROS-derived genetic instability and protein oxidation can also work in favor of pro-oncogenic signaling by modulating metabolic pathways and supporting neoplastic transformation 5,6,7