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For peripheral nerve analysis, a 5-mm-long piece of tail 15 mm distal from the tail base was collected from each sacrificed animal, fixed in 4% formaldehyde in phosphate buffer (pH 7.4), decalcinated, and impregnated with 1% osmium tetroxide for a few days

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32 Table 1

No matter the origin, in small amounts (up to 1%) its a nice, gentle preservative

pH curves for the two DHBA isomers are presented in Figure 3, which clearly illustrates the (de)protonation behavior of the DHBA molecules over the studied pH range, with the dissociation of the carboxylic group at acidic pH and the dissociation of the first hydroxyl group at basic pH clearly visible