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Abbreviations ACN: Acetonitrile AGC: Automatic gain control ARA: Arachidonic acid (20:4(5Z,8Z,11Z,14Z)) CE: Collision energy Chol Ester: Cholesteryl ester CXP: Collision cell exit potential dd: Data-dependent DG: Diacylglycerol DHA: Docosahexaenoic acid (22:6(4Z,7Z,10Z,13Z,16Z,19Z)) DP: Declustering potential EDTA: Ethylenediaminetetraacetic acid EPA: Eicosapentaenoic acid (20:5(5Z,8Z,11Z,14Z,17Z)) ESI: Electrospray ionization ESI( +): Positive electrospray ionization ESI(-): Negative electrospray ionization FC: Free cholesterol FWHM: Full width at half maximum HESI: Heated electrospray ionization HILIC: Hydrophilic interaction liquid chromatography HPLC: High-pressure liquid chromatography HRMS: High-resolution mass spectrometry IPA: iso -Propanol IS: Internal standard IT: Injection time LC: Liquid chromatography LLE: Liquid-liquid extraction LLOQ: Lower limit of quantification LOD: Limit of detection LPC: Lysophosphatidylcholine LPE: Lysophosphatidylethanolamine m/z : Mass-to-charge ratio MeOH: Methanol MG: Monoacylglycerol MRM: Multiple reaction monitoring MS: Mass spectrometry MTBE: tert -Butyl methyl ether NCE: Normalized collision energy NPLC: Normal-phase liquid chromatography PA: Phosphatidic acid PC: Phosphatidylcholine PE: Phosphatidylethanolamine PG: Phosphatidylglycerol PI: Phosphatidylinositol PS: Phosphatidylserine PUFA: Polyunsaturated fatty acid R: Resolution RF: Radio frequency RPLC: Reversed-phase liquid chromatography RSD: Relative standard deviation S / N : Signal-to-noise ratio SM: Sphingomyelin sn : Stereospecific numbering SPLASH: Single-vial prepared lipidomic analytical standard for human plasma lipids TG: Triacylglycerol TIC: Total ion chromatogram t R : Retention time XIC: Extracted ion chromatogram References Fahy E, Subramaniam S, Brown HA, Glass CK, Merrill AH, Murphy RC, et al

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