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Cells were then fixed with 4% paraformaldehyde, treated with 0.1% Triton X-100 in PBS for 10 min, incubated with 0.1% Tween 20, 0.3 M glycine, and 1% BSA in PBS for 2 h and incubated overnight at +4 C with monoclonal antibodies against -III tubulin conjugated to Alexa Fluor 647 (1:500, 657405, Biolegend, USA) in the same buffer

Additionally, the N-amino end of the peptide undergoes N-acetylation, and the C-carboxyl end of the peptide is amidated

doi: 10.1016/j.bbi.2016.03.010 193 VerstrepenL.BekaertT.ChauT