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Description
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Again, Romeos symptoms subsided for a few months
Tissue was then cryosectioned coronally at 20 C into 24 well plates (ThermoFisher Scientific) containing PBS 0.1% sodium azide at 25 m thickness and stored at 4 C until use
Obesity and type 2 diabetes (T2D) are themselves associated with higher rates of thyroid dysfunction and altered autoimmunity, underscoring the convergence of metabolic and endocrine pathways [5,6]
After three PBS washes, sections were incubated for 1 hour at RT with species-specific secondary antibodies: donkey anti-rabbit Alexa Fluor 594 (1:1000
