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The absorbance of each sample was monitored at 412 nm for 1 min, and the oxidized GSSG and total GSH (GSH + GSSG) levels were calculated using the standard curves of GSSG and GSH, respectively

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To determine the GSH concentration, a 200-l aliquot of the supernatant was added to 800 l of Na 2 HPO 4 solution (300 mmol/l)
The administration of NPs has been shown to cause severe developmental toxicity, as evidenced by spinal deformity and organ edema, through the activation of ferroptosis, apoptosis, and inflammation in zebrafish larvae

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