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However both enzymatic activities assayed at two different time points (24 hours and 5 days after plating) showed no differences between control and cells depleted of GSH with either BSO or DEM

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Additionally, there are other systems independent of GPX4 that regulate ferroptosis, such as ferroptosis suppressor protein 1 (FSP1)/ubiquinone (CoQ10) [23], dihydroorotate dehydrogenase (DHODH) [24], GTP cyclohydrolase-1 (GCH1)/tetrahydrobiopterin (BH4) [25], and amino acid oxidase interleukin-4-induced-1/indole-3-pyruvate [26]

Dysregulation of redox homeostasis leads to ROS accumulation, mitochondrial dysfunction, disrupted iron metabolism, and DNA damage, ultimately contributing to oxidative stress [168170]