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Validation of Primers Quantitative RT-PCR assays were initially optimized to give PCR efficiency between 1.9-2.1 (as determined by a 3-point standard curving using serial dilutions of control RNA with a slope range of 2.9 to 3.5) and a single melt-peak corresponding to the appropriate PCR product as verified by 2 % agarose gel electrophoresis

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For healthy athletes, however, evidence for broad improvements in body composition or aerobic performance is mixed

10.1038/ncomms7498 Summary Keywords gut microbiota, cardiovascular diseases, heart failure, metabolites, microbiota composition Citation Zhang Y, Wu H, Jin M, Feng G and Wang S (2025) The gut-heart axis: unveiling the roles of gut microbiota in cardiovascular diseases

Brief heat for mixing is not destructive