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Absorbance of culture media alone was considered as baseline

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Recent studies further demonstrate that vascular regression and impaired angiogenesis within the corpus luteum are strongly associated with hypoxia-driven ROS accumulation and subsequent luteal insufficiency [44]

The fix is switching the dose to before 10 AM
Glutathione transferase (GST) activity toward 1-chloro-2,4-dinitrobenzene (CDNB) was determined by spectrophotometry as described previously ( S- MOC was performed by mixing 20 L of 1 mM Tris-HCl (pH 7.5), 10 L of 20 mg bovine serum albumin (BSA) and 50 g of protein extract with 170 L of water
